The Eel Pond mRNAseq Protocol

author:Titus Brown, Camille Scott, and Leigh Sheneman.

Special thanks to Dr. Joshua Rosenthal for his help in developing this, and to Dr. Leslie Babonis for her feedback on various problems!

The tutorial:

• 0. Downloading and Saving Your Initial Data
• 1. Quality Trimming and Filtering Your Sequences
• 2. Applying Digital Normalization
• 3. Running the Actual Assembly
• BLASTing your assembled data
• 5. Building transcript families
• 6. Annotating transcript families
• 7. Expression analysis (with RSEM)
• 8. Differential expression (with EBSeq)

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LICENSE: This documentation and all textual/graphic site content is licensed under the Creative Commons - 0 License (CC0) -- fork @ github.

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